Technically, yes. Practically, no. The PC-10 is deceptively simple. Without the manual, here is what typically goes wrong:
A new manual is not just paperwork—it is a safety device. If your lab still uses a PC-10, print a fresh copy and laminate the calibration page next to the machine.
If you have a PC‑100 or PC‑100C, please specify — I can write a separate guide for those digital models.
Narishige PC-10 is a dual-stage vertical micropipette puller designed for high-reproducibility fabrication of microinjection needles and patch-clamp electrodes. While the Narishige PC-100
is the updated compatible replacement, the PC-10 remains a laboratory staple due to its robust manual controls. NARISHIGE GROUP Core Functionality & Operation Dual-Stage Pulling: narishige pc10 manual new
Automates the transition from a first "thinning" pull to a second final pull for consistent tip geometry. Stabilized Power Supply:
Features a built-in digital display of output voltage to minimize variation and improve reproducibility. Gravity-Based Pull:
Uses specific weight blocks (light and heavy) to vary the pulling force. Acrylic Shroud:
Includes a transparent cover to shield the glass capillaries from air currents during the heating process. NARISHIGE WEB NEWS Typical Settings for Common Pipettes Settings vary by glass type, but general rules apply: NARISHIGE WEB NEWS High Heater Value: Produces long, thin pipettes. Low Heater Value: Produces short, thick pipettes. Double Pull: Technically, yes
Typically yields "firm" pipettes suitable for patch-clamping or penetrative injections. NARISHIGE WEB NEWS Troubleshooting & Error Messages If your unit is displaying an error code, refer to the official Narishige FAQ for guidance:
Heater unit moving distance is set to 0mm; set to at least 1mm. First pull not detected; increase No. 1 heater output.
Slider descending too fast; reduce No. 1 heater output or adjust weights.
Poor contact or broken heater; tighten fixing screws or replace the filament. NARISHIGE GROUP Maintenance Tips A new manual is not just paperwork—it is a safety device
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The PC-10 has several mechanical components that wear out: the heater base, the solenoid plunger, the micro-switch, and the braking mechanism. A new manual should provide an exploded view with Narishige part numbers (e.g., for the heating filament or O-rings).
Step 1: Selecting the Glass The PC-10 is compatible with various glass capillaries (e.g., borosilicate, aluminosilicate).
Step 2: Installing the Glass
Step 3: Attaching the Weight