This book is a definitive graduate-level/advanced undergraduate resource. Core topics include:
Graphical Methods
Enzyme Inhibition
Multi-substrate Reactions
pH and Temperature Effects
Special Topics
[
v = \fracV_\textmax [S]K_m + [S]
]
Where:
– ( v ) = initial velocity
– ( V_\textmax = k_\textcat[E]T )
– ( K_m = \frack-1 + k_\textcatk_1 ) (steady-state) or ( K_m = K_s = \frack_-1k_1 ) (rapid equilibrium) Segel Enzyme Kinetics Pdf
You observe curvature at high substrate concentrations. Your colleagues say “just use the linear range.” Segel says: that is substrate inhibition. Turn to page 223 in the PDF. Use the equation ( v = \fracV_max1 + K_m/[S] + [S]/K_si ). He walks you through how to estimate ( K_si ) from a plot of ( 1/v ) vs. ( [S] ).